Related Experiment Video
Updated: Jun 20, 2026

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
Published on: January 4, 2017
Expression of extracellular matrix-proteins in perisellar connective tissue and dura mater
Ulrich J Knappe1, Thomas Fink, Annette Fisseler-Eckhoff
1Department of Neurosurgery, Johannes Wesling Klinikum, Hans-Nolte-Str. 1, 32429 Minden, Germany. ulrich.knappe@klinikum-minden.de
Purpose:
To describe the pattern of expression of extracellular matrix (ECM) proteins in perisellar connective tissue.
Methods:
Dural and perisellar specimens from ten individuals were investigated immunohistochemically for collagens I to IV, tenascin, fibronectin, elastin, laminin, and vitronectin.
Findings:
Collagen I and III and fibronectin were strongly expressed and collagen IV, tenascin, and vitronectin were moderately expressed in the boundaries of the sella and around the CS. In six of nine specimens from the anterior boundary of the sella, and in 11 of 19 samples from the lateral boundary of the sella (medial wall of CS), two different layers could be detected by the expression of different ECM proteins. None of the antigens generally allowed differentiation between two layers of the pituitary envelope.
Conclusions:
The pituitary boundary may consist of a single or a double layer, infrequently differentiated from each other by the expression of different ECM proteins.
Related Concept Videos
Extracellular Matrix
The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
The Extracellular Matrix
Matrix Proteoglycans and Glycoproteins
Connective Tissue Fibers and Ground Substance
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...

