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Updated: Jun 20, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
An imidazopyridinone and further metabolites from streptomycetes
Hafizur Rahman1, Mohamed Shaaban, Khaled A Shaaban
1Institute of Organic and Biomolecular Chemistry, University of Göttingen, Tammannstrasse 2, D-37077 Göttingen, Germany.
Abstract:
Screening and chromatographic analysis of the extracts from the terrestrial streptomycete GW5127 and from the marine Streptomyces sp. isolate B7967 yielded four new metabolites, i.e. 8-ethyl-6,11-dihydroxy-l1-methoxy-naphthacene-5,12-dione (la), 4-acetyl-1,3-dihydro-imidazo[4,5-c]pyridin-2-one (4a), 5-hydroxmethyl-4-hydroxy-2,4-dimethyl-2-cyclopentenone (6), and N-[2-(3'-hydroxy-4'-methoxyphenyl)-ethyl]-acetamide (5a), along with five known compounds, beta-rubromycin (2), rubromycin (3), 2-(3'-hydroxy-4'-methoxyphenyl)ethanol (5b), uracil, and N-acetyltyramine. The structures of the new compounds were deduced from high resolution mass, 1D and 2D NMR spectra and by comparison with related compounds from the literature. The antibiotic activity of the crude extracts was due to 2 and 3.
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