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SN2 Reaction: Stereochemistry

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In an SN2 reaction, the nucleophilic attack on the substrate and departure of the leaving group occurs simultaneously through a transition state. As the nucleophile approaches the substrate from the back-side, the configuration of the substrate carbon changes from tetrahedral to trigonal bipyramidal and then back to tetrahedral, leading to an inversion in the configuration of the product.
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SN1 Reaction: Stereochemistry

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Related Experiment Video

Updated: Jun 20, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

SRC substrate surprise.

G Steven Martin¹

¹Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, Berkeley, Berkeley, CA 94720, USA. gsm@berkeley.edu

|September 8, 2009

Summary

Tensin-3

Area of Science:

Oncology
Molecular Biology
Cell Signaling

Background:

Tensin-3 is implicated in cancer progression.
Regulation of tensin-3 activity is crucial for understanding its role in oncogenesis.

Purpose of the Study:

To investigate the regulatory mechanisms of tensin-3.
To elucidate how Src influences tensin-3 function.

Main Methods:

Phosphorylation assays
Analysis of SH2 domain function
Studies on oncogenic activity

Main Results:

Src phosphorylates the SH2 domain of tensin-3.
This phosphorylation enhances the oncogenic function of tensin-3.

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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Published on: October 31, 2014

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Last Updated: Jun 20, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

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Published on: September 20, 2016

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Substrate Generation for Endonucleases of CRISPR/Cas Systems

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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Published on: October 31, 2014

Phosphorylation regulates SH2 ligand binding.

Conclusions:

Src-mediated phosphorylation of the tensin-3 SH2 domain is a novel regulatory mechanism.
This mechanism contributes to the oncogenic potential of tensin-3.