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Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
Chromatographic Resolution01:15

Chromatographic Resolution

In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Spatially resolved multidimensional cross-correlation relaxometry.

Luca Venturi1, Brian Hills

  • 1Institute of Food Research, MRI-Exploitation Platform, Norwich Research Park, Colney, Norwich, NR47UA Norfolk, UK.

Magnetic Resonance Imaging
|September 8, 2009
PubMed
Summary
This summary is machine-generated.

New methods allow for detailed relaxation time spectra analysis in heterogeneous samples. These techniques are optimized for accessible, low-field bench-top relaxometers, broadening research capabilities.

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A Multimodal Wide-Field Fourier-Transform Raman Microscope
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A Multimodal Wide-Field Fourier-Transform Raman Microscope

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Published on: December 11, 2021

A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Materials Science

Background:

  • Macroscopically heterogeneous samples present challenges for traditional relaxation time measurements.
  • Existing techniques often require high-field NMR instruments, limiting accessibility.

Purpose of the Study:

  • To present novel protocols for acquiring one- and two-dimensional relaxation time spectra.
  • To adapt these protocols for implementation on low-cost, low-field bench-top relaxometers.

Main Methods:

  • Development of specialized pulse sequences for selective subvolume analysis.
  • Adaptation of protocols for instruments lacking advanced features like pulse shaping or pulsed field gradients.

Main Results:

  • Successful acquisition of one- and two-dimensional relaxation time spectra from selected subvolumes.
  • Demonstration of the general applicability of the protocols across different sample types.

Conclusions:

  • The presented protocols enable detailed relaxation analysis in heterogeneous samples using accessible instrumentation.
  • These methods significantly lower the barrier to entry for advanced NMR relaxation studies.