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Human bocavirus and rhino-enteroviruses in childhood otitis media with effusion

Szilárd Rezes1, Maria Söderlund-Venermo, Merja Roivainen

  • 1Department of Otorhinolaryngology and Head and Neck Surgery, Health Science Centre, University of Debrecen, 98 Nagyerdei krt., Debrecen, Hungary. szrezes@gmail.hu

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|September 9, 2009
PubMed
Abstract

Insights

Human enteroviruses (HEV) and human rhinoviruses (HRV) were detected in middle ear effusions of children with otitis media with effusion (OME). Human bocavirus (HBoV) was also found, suggesting these viruses may be associated with OME.

Area of Science:

  • Otolaryngology
  • Pediatric Infectious Diseases
  • Virology

Background:

  • Otitis media with effusion (OME) in children is often linked to viral respiratory infections.
  • Human rhinoviruses (HRVs) are common in middle ear effusions (MEE), but data on human enteroviruses (HEVs) and human bocavirus (HBoV) are limited.
  • HBoV has not previously been identified in MEE from OME patients.

Purpose of the Study:

  • To determine the presence and coinfection rates of HBoV and HRV/HEV in MEE samples from children with OME.
  • To investigate the association of these viruses with the pathogenesis of OME.

Main Methods:

  • Seventy-five MEE samples from 54 children without acute respiratory symptoms were analyzed.
  • Reverse transcription polymerase chain reaction (RT-PCR) was used for HRV/HEV detection.
  • Quantitative PCR was employed for HBoV detection.

Main Results:

  • Viral nucleic acid was detected in 35% of MEE samples.
  • HEV was found in 29%, HRV in 13%, and HBoV in 3% of samples.
  • No significant difference in virus detection rates was observed between mucoid and serous effusions; 43% of bilateral cases showed contralateral viral differences.

Conclusions:

  • The study suggests common respiratory viruses, including HEV, HRV, and HBoV, are associated with OME in children.
  • The etiological role of these viruses in persistent MEE requires further investigation.
  • Distinguishing between causative factors and remnants of past infections is crucial.

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