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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

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Published on: December 23, 2020

[Study on interaction between SARS-CoV N and MAP19].

Jun-li Liu1, Cheng Cao, Qing-jun Ma

  • 1Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China. liujunli306@sohu.com

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|September 10, 2009
PubMed
Summary
This summary is machine-generated.

The nucleocapsid (N) protein of SARS-CoV interacts with MAP19. This study demonstrates SARS-CoV N binding to MAP19 in cells, potentially aiding future research.

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Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) causes SARS, an emerging atypical pneumonia.
  • The nucleocapsid (N) protein of SARS-CoV plays a key role in viral replication.
  • MAP19 is a non-enzymatic protein associated with mannan-associated serine protease (MASP).

Purpose of the Study:

  • To investigate the interaction between the SARS-CoV N protein and MAP19.
  • To determine if SARS-CoV N influences the amount of MAP19 in cells.

Main Methods:

  • Yeast two-hybrid screen to identify interacting proteins.
  • Immunoprecipitation to confirm protein-protein interactions.
  • Western blot to quantify protein levels.

Main Results:

  • The SARS-CoV N protein was found to interact with MAP19.
  • Immunoprecipitation confirmed the interaction between SARS-CoV N and MAP19.
  • SARS-CoV N significantly increased the cellular amount of MAP19.

Conclusions:

  • SARS-CoV N protein binds to MAP19 within host cells.
  • This interaction may be relevant to the molecular mechanisms of SARS-CoV pathogenesis.
  • Further research into the SARS-CoV N-MAP19 interaction is warranted.