Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transverse Force Distributions in the Proton from Lattice QCD.

Physical review letters·2025
Same author

Surface Roughness Studies with DALLAS-Detector Array for Laser Light Angular Scattering.

Journal of research of the National Bureau of Standards (1977)·2021
Same author

The effect of bacteriochlorophyll derivative WST-D and near infrared light on the molecular and fibrillar architecture of the corneal stroma.

Scientific reports·2020
Same author

Accelerating Lattice Quantum Field Theory Calculations via Interpolator Optimization Using Noisy Intermediate-Scale Quantum Computing.

Physical review letters·2020
Same author

Nucleon Structure Functions from Operator Product Expansion on the Lattice.

Physical review letters·2017
Same author

Determination of the strange nucleon form factors.

Physical review letters·2015
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

Scanning tunneling microscopy applied to optical surfaces.

R A Dragoset, R D Young, H P Layer

    Optics Letters
    |September 10, 2009
    PubMed
    Summary
    This summary is machine-generated.

    Scanning tunneling microscopy (STM) successfully mapped the topography of optical surfaces, revealing nanoscale details on a ruled grating replica and a gold mirror. This advanced technique offers unprecedented insights into surface structures crucial for understanding precision manufacturing processes.

    More Related Videos

    Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
    10:28

    Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

    Published on: May 27, 2018

    Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry
    08:18

    Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry

    Published on: March 4, 2021

    Related Experiment Videos

    Last Updated: Jun 20, 2026

    All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
    11:33

    All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

    Published on: January 19, 2018

    Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
    10:28

    Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

    Published on: May 27, 2018

    Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry
    08:18

    Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry

    Published on: March 4, 2021

    Area of Science:

    • Surface Science
    • Nanotechnology
    • Metrology

    Background:

    • Accurate topographic mapping of optical surfaces is critical for quality control and understanding material properties.
    • Existing surface-sensitive microscopes have limitations in resolving fine surface structures.
    • Diamond-turning is a precision manufacturing process requiring detailed surface analysis.

    Purpose of the Study:

    • To demonstrate the capability of Scanning Tunneling Microscopy (STM) for high-resolution topographic mapping of optical surfaces.
    • To assess STM's effectiveness in characterizing ruled grating replicas and diamond-turned gold mirrors.
    • To reveal surface structures undetectable by conventional microscopy techniques.

    Main Methods:

    • Application of Scanning Tunneling Microscopy (STM) for surface topography measurements.
    • Focused on a ruled grating replica and a diamond-turned gold mirror.
    • Detailed nanoscale imaging over a 2 microm x 2 microm area.

    Main Results:

    • STM successfully measured the surface topography of a ruled grating replica with high resolution.
    • Unprecedented surface structures on a diamond-turned gold mirror were observed using STM.
    • These structures were not detectable with other surface-sensitive microscopy methods.

    Conclusions:

    • STM is a powerful tool for detailed topographic mapping of optical surfaces.
    • The technique provides critical information for understanding the diamond-turning process.
    • STM enables the discovery of previously unobserved surface features, advancing metrology and manufacturing insights.