A simple parametric method for reducing sample sizes in gut passage time trials
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Updated: Jun 20, 2026

High-throughput Measurement of Gut Transit Time Using Larval Zebrafish
Published on: October 23, 2018
John Rawsthorne1, David A Roshier, Sean R Murphy
1Institute for Land, Water and Society, Charles Sturt University, P.O. Box 789, Albury, NSW 2640, Australia.
Abstract:
Seed dispersal is now regularly analyzed using spatially explicit models, relying in part on frugivore gut passage times to produce model outputs. In determining species-specific gut passage times, there is a trade-off in sample size between minimizing collection effort and maintaining statistical reliability. Here we demonstrate that a two-parameter lognormal parametric distribution reliably fits empirical gut passage time distributions and is easily parameterized using relatively small data sets of approximately 30 defecations. We suggest this approach as a statistically reliable substitute for larger empirical gut passage data sets in seed dispersal modeling, and also as a way of using published gut passage data sets to parameterize new models.

