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Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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Implications of chimaeric non-co-linear transcripts.

Thomas R Gingeras1

  • 1Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA. gingeras@cshl.edu

Nature
|September 11, 2009
PubMed
Summary
This summary is machine-generated.

Researchers discovered non-co-linear transcripts, known as chimaeric RNAs, in organisms from worms to humans. These transcripts, formed by genetic or post-transcriptional events, challenge our understanding of genome information content.

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Published on: April 30, 2011

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07:35

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries

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Area of Science:

  • Genomics
  • Molecular Biology
  • RNA Biology

Background:

  • Deep sequencing reveals transcriptomes, the complete set of RNA transcripts.
  • Some transcripts exhibit non-co-linear structures, with segments from distant DNA regions.

Purpose of the Study:

  • Investigate the origin and implications of non-co-linear transcripts.
  • Understand the mechanisms behind chimaeric RNA formation in higher eukaryotes.

Main Methods:

  • Deep sequencing of transcriptomes across diverse species.
  • Analysis of RNA sequence composition and genomic origin.

Main Results:

  • Identified chimaeric transcripts composed of non-contiguous DNA segments.
  • Observed chimaeric RNAs arising from both genetic rearrangements and post-transcriptional events.
  • Highlighted the limited understanding of trans-splicing in higher eukaryotes.

Conclusions:

  • Chimaeric RNAs are a significant feature across the animal kingdom.
  • These findings expand the known complexity of genome information and its arrangement.
  • Further research is needed to elucidate chimaeric RNA formation in higher eukaryotes.