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Updated: Jun 20, 2026

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
Published on: November 11, 2017
Imaging chiroptical artifacts
John H Freudenthal1, Eva Hollis, Bart Kahr
1Department of Chemistry, University of Washington, Seattle, Washington, USA.
Abstract:
It is well-known that circular dichroism (CD) measurements of anisotropic media may contain artifacts that result from mixed linear anisotropies. Such artifacts are generally considered a nuisance. However, systematic artifacts, carefully measured, may contain valuable information. Herein, polycrystalline spherulites of D-sorbitol grown from the melt were analyzed with a Mueller matrix microscope, among other differential polarization images devices. As spherulites grew into one another they developed strong apparent optical rotation and CD signals at the boundaries between spherulites. These signals are shown not to have a chiroptical origin but rather result from the interactions of linear anisotropies in polycrystalline bodies. Such chiroptical artifacts should not be dismissed reflexively. Rather, they are comprehensible crystal-optical effects that serve to define mesoscale structure.
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