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The mammalian anti-proliferative BTG/Tob protein family

G Sebastiaan Winkler1

  • 1School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, United Kingdom. sebastiaan.winkler@nottingham.ac.uk

Journal of Cellular Physiology
|September 12, 2009
PubMed

Insights

The BTG/Tob protein family regulates cell cycle and gene expression. These proteins interact with transcription factors and Ccr4-Not complex components, impacting mRNA turnover and potentially cancer development.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The mammalian BTG/Tob protein family consists of six members involved in cell cycle regulation across various cell types.
  • These proteins share a conserved N-terminal BTG domain (104-106 amino acids) crucial for protein-protein interactions.

Purpose of the Study:

  • To review the human and mouse BTG/Tob proteins.
  • To discuss structural aspects of BTG/Tob interactions with Ccr4-Not complex components.
  • To elucidate the role of BTG/Tob proteins in gene expression regulation, tumorigenesis, and cancer.

Main Methods:

  • Biochemical and structural data analysis.
  • Review of existing literature on BTG/Tob protein functions.
  • Focus on interactions with DNA-binding transcription factors and Ccr4-Not complex subunits (CNOT7, CNOT8).

Main Results:

  • The conserved BTG domain acts as a protein-protein interaction module.
  • BTG/Tob proteins bind to transcription factors and Ccr4-Not deadenylase subunits.
  • Members are involved in nuclear transcription and cytoplasmic mRNA deadenylation/turnover.
  • Less conserved C-terminal regions mediate unique interactions.

Conclusions:

  • BTG/Tob proteins are key regulators of gene expression through interactions with transcription and mRNA decay machinery.
  • Dysregulation of BTG/Tob proteins may contribute to tumorigenesis and cancer progression.

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