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Updated: Jun 20, 2026

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
Published on: September 2, 2014
DcR3 protects THP-1 macrophages from apoptosis by increasing integrin alpha4
Koji Tateishi1, Yasushi Miura, Shinya Hayashi
1Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Abstract:
In order to analyze the function of DcR3 for the regulation of cell adhesion and apoptosis in macrophages, we investigated the expression of decoy receptor 3 (DcR3) in THP-1 monocytes/macrophages. DcR3 was expressed in THP-1 and increased by phorbol 12-myristate 13-acetate (PMA). The formation of macrophage aggregates was observed when THP-1 cells were differentiated by PMA or stimulated with DcR3-Fc. Undifferentiated THP-1 cells were also induced to form aggregates by DcR3-Fc. The expression of integrin alpha4 was significantly increased by DcR3-Fc. CHX-induced apoptosis in THP-1 was inhibited by DcR3-Fc, of which inhibition against CHX-induced apoptosis and aggregate formation were ameliorated by anti-VLA4 antibody. DcR3 may play a significant role in macrophages not only by a decoy receptor but also by increasing alpha4 integrin.
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