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Updated: Jun 20, 2026
An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection
Published on: June 30, 2023
A novel convenient method for high bacteriophage titer assay
Kazuo Yoshida1, Yuki Nasu, Nozomi Shitami
1Department of Nutritional Sciences, Yasuda Women's University, Hiroshima 731-0153, Japan. kazuyosi@hiroshima-u.ac.jp
Abstract:
The recent various applications of phages (bacteriophages) including phage therapy have brought about a revival of phage investigation. The phage titer assay is indispensable for phage experiments. However, the conventional standard method is a plaque counting method which requires a little skill with tedious repeating operation. Furthermore, it is not directly applicable to high phage titers. In this paper, we describe a novel convenient "cross streak and paper disk assay method" for high titer concentration without plaque counting.
