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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Interference and Diffraction02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Continuous -time Fourier Transform01:11

Continuous -time Fourier Transform

The Fourier series is instrumental in representing periodic functions, offering a powerful method to decompose such functions into a sum of sinusoids. This technique, however, necessitates modification when applied to nonperiodic functions. Consider a pulse-train waveform consisting of a series of rectangular pulses. When these pulses have a finite period, they can be accurately represented by a Fourier series. Yet, as the period approaches infinity, resulting in a single, isolated pulse, the...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 20, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Interferometric tomography of continuous fields with incomplete projections.

S S Cha, H Sun

    Optics Letters
    |September 15, 2009
    PubMed
    Summary

    This study presents an iterative algorithm for interferometric tomography that reconstructs object fields without needing extra information. The method focuses on the difference field, ensuring stable convergence even with opaque objects.

    Area of Science:

    • Optics and Photonics
    • Image Reconstruction
    • Wave Phenomena

    Background:

    • Interferometric tomography is a powerful imaging technique.
    • Reconstructing objects with opaque regions presents significant challenges due to missing data.
    • Existing methods often require data augmentation or assumptions about the missing information.

    Purpose of the Study:

    • To investigate interferometric tomography in the presence of opaque objects.
    • To develop an iterative algorithm capable of handling missing data without augmentation.
    • To demonstrate stable convergence of the proposed reconstruction method.

    Main Methods:

    • Developed an iterative algorithm for interferometric tomography.
    • The algorithm reconstructs the difference field between the object and its estimate.

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  • Reconstruction is performed only within a defined region, avoiding augmentation of missing data.

    • Main Results:

    • The iterative algorithm successfully reconstructs object fields in the presence of opaque regions.
    • The method does not require augmentation of missing information.
    • Stable convergence was achieved during the application of the algorithm.

    Conclusions:

    • The developed iterative algorithm offers a robust solution for interferometric tomography with opaque objects.
    • This approach overcomes limitations of methods requiring data augmentation.
    • The algorithm provides stable and reliable reconstructions, advancing the field of tomographic imaging.