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Updated: Jun 20, 2026

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
Published on: February 2, 2022
Walking, hopping, diffusing and braking modes of kinesin-5
Kuniyoshi Kaseda1, Andrew D McAinsh, Robert A Cross
1Molecular Motors Laboratory, Marie Curie Research Institute, Trevereux Hill, Limpsfield Chart, Oxted, Surrey, UK. k.kaseda@mcri.ac.uk
Abstract:
It is clear that the main cellular mission of the molecular motor kinesin-5 (known as Eg5 in vertebrates) is to cross-link antiparallel microtubules and to slide them apart, thus playing a critical role during bipolar spindle formation. Nonetheless, important questions about the cell biological and biophysical mechanisms of Eg5 remain unanswered. With the 20th 'birthday' of Eg5 approaching, we discuss recent insights into the in vitro and in vivo functions of Eg5, in the context of our own recent work.
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