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Updated: Jun 20, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Controlling depth of focus in 3D image reconstructions by flexible and adaptive deformation of digital holograms
P Ferraro1, M Paturzo, P Memmolo
1CNR-Istituto Nazionale di Ottica Applicata and Istituto di Cibernetica, via Campi Flegrei 34,80078 Pozzuoli (NA), Italy. pietro.ferraro@inoa.it
Abstract:
We show here that through an adaptive deformation of digital holograms it is possible to manage the depth of focus in 3D imaging reconstruction. Deformation is applied to the original hologram with the aim to put simultaneously in focus, and in one reconstructed image plane, different objects lying at different distances from the hologram plane (i.e., CCD sensor). In the same way, by adapting the deformation it is possible to extend the depth of field having a tilted object entirely in focus. We demonstrate the method in both lensless as well as in microscope configuration.

