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Updated: Jun 20, 2026

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A Protocol for Housing Mice in an Enriched Environment
Published on: June 8, 2015
Shelter enrichment for rats
1Department of Behavioural Sciences, Animal Biology Division, Sir Stephen Watson Building, Bush Estate, Penicuik EH26 0PH, Scotland, UK.
Summary
Providing nest boxes is crucial for environmental enrichment in laboratory rats. Rats show a strong preference for enclosed, opaque, thermoplastic nest boxes, which should be implemented in all research facilities.
Area of Science:
- Animal behavior
- Laboratory animal science
- Environmental enrichment
Background:
- Nest boxes are a common method for environmental enrichment in laboratory animal research.
- Providing appropriate housing conditions is essential for animal welfare and research integrity.
- Understanding animal preferences guides the selection of enrichment tools.
Purpose of the Study:
- To evaluate rat preferences for different types of nest boxes.
- To determine the most preferred nest box characteristics for laboratory rats.
- To provide recommendations for nest box implementation in research settings.
Main Methods:
- Rats were presented with various nest box designs.
- T-maze tasks were utilized to assess nest box preference.
- Preference was measured by T-maze choice.
Main Results:
- Rats demonstrated acceptance of a wide variety of nest box types.
- A significant preference was observed for enclosed, opaque, thermoplastic nest boxes.
- Nest box design significantly influences rat choices.
Conclusions:
- Nest boxes are a valuable and effective environmental enrichment for laboratory rats.
- Enclosed, opaque, thermoplastic nest boxes are strongly preferred by rats.
- Implementation of preferred nest box types should be considered in all laboratory animal facilities.
