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Published on: March 27, 2017
Detection of angiotensin II type 1 receptor ligands by a cell-based assay
Matthias Griessner1, Patrick Bröker, André Lehmann
1Fraunhofer Institute for Biomedical Engineering (IBMT), Am Mühlenberg 13, 14476 Potsdam, Germany. matthias.griessner@ibmt.fraunhofer.de
Abstract:
This work describes a cell-based assay that does not depend on radioactivity or laboratory animals for the detection of ligands of angiotensin II type 1 receptor (AT1R). The assay makes use of stable transfected Chinese hamster ovary cells (CHO-AT1R) expressing the AT1R. A sequential saturation assay principle was used in which receptor binding sites of the CHO-AT1R cells are blocked by the analyte in a concentration-dependent manner. Afterwards, TAMRA-angiotensin II, a fluorescence-labeled ligand, was added to bind to the remaining free binding sites of the receptor. In consequence, the fluorescence signal determined is inversely proportional to the concentration of the analyte.
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