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Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

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Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

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A switchable light-input, light-output system modelled and constructed in yeast.

Oxana Sorokina¹, Anita Kapus², Kata Terecskei²

¹Institute of Molecular Plant Sciences, The University of Edinburgh, Kings Buildings, Mayfield Road, Edinburgh EH9 3JH, UK.

Journal of Biological Engineering

|September 19, 2009

Summary

Scientists created a novel light-switchable system in yeast using plant proteins. This synthetic biology tool enables dynamic, predictable control over gene expression with light, advancing genetic network regulation.

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Area of Science:

Synthetic Biology
Molecular Biology
Biotechnology

Background:

Synthetic biology demands precise spatio-temporal control of biological processes within host cells.
Existing methods often lack the dynamic range required for complex genetic network regulation.

Purpose of the Study:

To develop a novel, light-inducible regulatory system for eukaryotic hosts.
To enable dynamic and predictable control of gene expression using light signals.

Main Methods:

Engineered a light-switchable, two-hybrid interaction system in yeast using Arabidopsis PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE proteins.
Utilized a LUCIFERASE reporter gene for real-time detection of gene expression via imaging.
Developed a mathematical model based on quantitative readout to predict system behavior.

Main Results:

Demonstrated reversible light-dependent activation (red light) and inactivation (far-red light) of the engineered phytochrome system.
Achieved dynamic control over the LUCIFERASE reporter gene expression.
Constructed a predictive mathematical model of the system's response to light stimuli.

Conclusions:

The developed system offers a novel method for converting dynamic light input into predictable gene expression responses in eukaryotic hosts.
This platform has significant potential for regulating both known and synthetic genetic networks.
The integrated model, methods, and materials provide a versatile tool for synthetic biology applications.