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Related Experiment Video

Updated: Jun 20, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

HPLC/MS/MS-based approaches for detection and quantification of eicosanoids.

Susanna L Lundström1, Fabio L D'Alexandri, Kasem Nithipatikom

  • 1Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, Stockholm, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
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Quantifying eicosanoids, crucial in diseases like asthma and cancer, requires sensitive methods. This study details High-Performance Liquid Chromatography Mass Spectrometry (HPLC/MS) techniques for accurate eicosanoid analysis in biological samples.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomedical Science

Background:

  • Eicosanoids are endogenous unsaturated fatty acids derived from arachidonic acid.
  • These compounds play significant roles in diseases such as asthma, COPD, cardiovascular disease, and cancer.
  • Low endogenous levels necessitate sensitive and specific analytical methods for reliable quantification.

Purpose of the Study:

  • To describe High-Performance Liquid Chromatography Mass Spectrometry (HPLC/MS) techniques and principles for eicosanoid analysis.
  • To provide a protocol for extracting eicosanoids from biological samples, exemplified by bronchoalveolar lavage fluid (BALF).
  • To offer an introduction to methodologies for eicosanoid quantification.

Main Methods:

  • Utilized High-Performance Liquid Chromatography Mass Spectrometry (HPLC/MS) for eicosanoid profiling.

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  • Developed and optimized a protocol for eicosanoid extraction from biological samples (BALF).
  • Employed reverse-phase HPLC interfaced with an ion trap mass spectrometer (LC/MS/MS) for standard analysis.

    • Main Results:

    • Presented key LC/MS techniques and principles applicable to eicosanoid analysis.
    • Detailed a comprehensive protocol for eicosanoid extraction and sample preparation.
    • Demonstrated the analysis of eicosanoid standards using LC/MS/MS, validating the methodology.

    Conclusions:

    • HPLC/MS is a powerful technique for sensitive and specific eicosanoid quantification.
    • The provided protocol facilitates eicosanoid analysis in biological matrices like BALF.
    • This work serves as an introductory guide to essential methodologies in eicosanoid profiling.