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Transgenic Organisms00:53

Transgenic Organisms

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jun 20, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Transgenic mouse technology: principles and methods.

T Rajendra Kumar1, Melissa Larson, Huizhen Wang

  • 1Department of Molecular & Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary

Transgenic mouse technology allows introducing foreign DNA into mice, revolutionizing biology and disease modeling. The Cre-lox system offers precise gene control for studying developmental processes.

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Related Experiment Videos

Last Updated: Jun 20, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
10:21

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Endocrinology

Background:

  • Transgenic mouse technology enables foreign DNA introduction into the germ line, a significant advancement.
  • This technology has transformed biological research and human disease modeling in vivo.
  • Hundreds of transgenic lines targeting specific gene expression have been developed.

Purpose of the Study:

  • To discuss the principles of transgenic mouse technology.
  • To describe standardized methodologies for creating transgenic mice.
  • To highlight the application of Cre-lox technology in developmental studies.

Main Methods:

  • Germ line modification via foreign DNA introduction.
  • Generation and characterization of transgenic mouse lines.
  • Spatio-temporal gene expression control using Cre-lox technology.

Main Results:

  • Transgenic mice provide powerful genetic models for biological research.
  • Cre-lox technology allows precise in vivo gene manipulation.
  • Established methodologies facilitate the creation of specific transgenic models.

Conclusions:

  • Transgenic mouse technology is a cornerstone of modern biological and genetic research.
  • The Cre-lox system is crucial for dissecting complex developmental processes.
  • Standardized protocols enhance the efficiency and reliability of transgenic mouse generation.