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Updated: Jun 20, 2026
11:19
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
From spin-labeled proteins to in vivo EPR applications
1Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA. berliner@du.edu
European Biophysics Journal : EBJ
|September 19, 2009
Summary
This historical overview details the development of spin labeling for biological studies. It covers early applications and in vivo electron paramagnetic resonance (EPR) in living systems from a personal viewpoint.
Area of Science:
- Biophysics
- Biochemistry
- Medical Imaging
Background:
- Spin labeling is a technique used to study biological molecules.
- Electron paramagnetic resonance (EPR) is a spectroscopic method.
- The author was a Ph.D. student when spin labeling was conceived.
Purpose of the Study:
- To provide a historical overview of spin labeling applications in biological systems.
- To describe the development of in vivo EPR applications in living organisms.
- To share a personal perspective on the evolution of these techniques.
Main Methods:
- Historical review of scientific literature.
- Personal recollections and experiences.
- Focus on the advent and development of spin labeling and in vivo EPR.
Main Results:
- The advent and evolution of spin labeling in biological research.
- The progression of in vivo EPR applications in animal and living systems.
- A firsthand account of key developmental stages.
Conclusions:
- Spin labeling has significantly advanced biological studies.
- In vivo EPR has become a valuable tool for studying living systems.
- Personal involvement provided unique insights into technique development.
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