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[Study on purification and property of polysaccharide from Scolopendra subspinipes mutilans]
Xing-Nuan Li1, Xiang Xiao, Yun Wang
1College of Medical, Jiujiang University, Jiujiang 332000, China. li_xingnuan@126.com
Objective:
To obtain polysaccharide from Scolopendra subspinipes mutilans and study its partial properties.
Methods:
A raw polysaccharide was isolated from Chinese medicine Scolopendra subspinipes mutilans, and three groups were isolated by DEAE-52. The thin layer chromatography and gel filtration chromatography were used to detect the main monosaccharide composition and molecular weight of the group I. The inhibitory effect of the group I on tumor cells was detected by MTT assay.
Results:
The molecular weight of the group I was 33.1 kD. The inhibitory effect of the group I on Hela cells was obvious, as its inhibitive rate was 60.8% on Hela cells when the polysaccharide's concentration was 3.13 microg/mL, but it had no effect on the Eca 109 cells.
Conclusion:
The group I has specific effects on different tumor cells.
