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Related Concept Videos

Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
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[Novel human embryonic stem cell lines C612 and C910].

I V Kozhukharova, I I Fridlianskaia, Z V Kovaleva

    Tsitologiia
    |September 22, 2009
    PubMed
    Summary

    Two novel human embryonic stem cell lines, C612 and C910, have been established. These diploid stem cells express pluripotent markers and differentiate into three germ layers, showing potential for regenerative medicine.

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    Published on: July 7, 2023

    Area of Science:

    • Stem Cell Biology
    • Developmental Biology

    Context:

    • Human embryonic stem cells (hESCs) are crucial for understanding early development and disease.
    • Establishing new hESC lines with stable pluripotent characteristics is essential for research.

    Purpose:

    • To report the establishment and characterization of two novel human embryonic stem cell lines, C612 and C910.
    • To confirm their pluripotent nature and suitability for future research.

    Summary:

    • Novel human embryonic stem cell lines, C612 and C910, were derived from hatching blastocysts.
    • Cells express key pluripotent markers (Oct 3/4, Nanog, alkaline phosphatase) and surface antigens (CD90, CD117).
    • Karyotypic analysis confirmed normal diploid status, and differentiation potential into all three germ layers was demonstrated.

    Impact:

    • These cell lines possess typical human embryonic stem cell attributes, including self-renewal and differentiation capacity.
    • C612 and C910 lines offer valuable tools for fundamental research and regenerative medicine applications.