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Updated: Jun 20, 2026

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
Published on: January 30, 2016
A model based atomic resolution tomographic algorithm
W Van den Broek1, S Van Aert, D Van Dyck
1EMAT, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. wouter.vandenbroek@ua.ac.be
Abstract:
Tomography with high angular annular dark field scanning transmission electron microscopy at atomic resolution can be greatly improved if one is able to take advantage of prior knowledge. In this paper we present a reconstruction technique that explicitly takes into account the microscope parameters and the atomic nature of the projected object. This results in a more accurate estimate of the atomic positions and in a good resistance to noise. The reconstruction is a maximum likelihood estimator of the object. Moreover, the limits to the precision have been explored, allowing for a prediction of the amount of expected noise in the reconstruction for a certain experimental setup. We believe that the proposed reconstruction technique can be generalized to other tomographic experiments.
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