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Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Comparison of nucleic acid extraction methods for the detection of Mycoplasma pneumoniae
Kathleen A Thurman1, Kelley C Cowart, Jonas M Winchell
1Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Diagnostic Microbiology and Infectious Disease
|September 22, 2009
Abstract:
Four nucleic acid extraction procedures (2 automated and 2 manual) were compared for their efficiency at isolating Mycoplasma pneumoniae DNA. Oropharyngeal swabs from healthy volunteers were spiked with varying amounts of M. pneumoniae, extracted, and tested using real-time polymerase chain reaction. Our data indicate that both automated extraction methods consistently outperform the manual procedures.
