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Updated: Jun 20, 2026
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Zn(II)-cyclen polyacrylamide gel electrophoresis for SNP detection
Emiko Kinoshita-Kikuta1, Eiji Kinoshita, Tohru Koike
1Department of Functional Molecular Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Abstract:
We introduce a method for the detection of single-nucleotide polymorphisms (SNPs) by polyacrylamide gel electrophoresis (PAGE) with an additive Zn2+-cyclen complex (cyclen is 1,4,7,10-tetraazacyclododecane), called "Zn2+-cyclen-PAGE." The method is based on the difference in mobility of mutant DNA (of the same length) in PAGE, which is due to Zn2+-cyclen binding to thymine bases accompanying a total charge decrease and a local conformation change of target DNA. In combination with a polymerase chain reaction based heteroduplexing technique, the method is more accurate than many other conventional PAGE-based methods, as shown by clear gel-shifting bands because of the separation of heteroduplex and homoduplex DNAs. We demonstrate SNP mapping and heterozygosity screening in a human cardiac sodium channel gene (i.e., SCN5A) that relates to inherited arrhythmia syndromes using Zn2+-cyclen-PAGE.
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