Related Experiment Video
Updated: Jun 20, 2026

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
Published on: September 28, 2022
Uber infektiöse Substrukturen aus Bakteriophagen. VI. A simple method for separating the replicative form from
R Benzinger1, R Jaenisch, P H Hofschneider
1Max-Planck-Institut für Biochemie Goethestrasse 31, Munich, Germany.
Abstract:
A selective adsorption method is presented which allows a rapid separation of phiX174 replicative form DNA from single-stranded phiX174 phage DNA, facilitating the handling of a large number of samples. The single-stranded phiX174 DNA is selectively adsorbed to silicic acid coated with methylated albumin. After removal of the adsorbent by sedimentation, at least 40% of the input replicative form DNA is recovered in the supernatant fraction; the ratio of replicative form to single-stranded phage DNA is increased 20- to 200-fold. The potential of the method for separating other nucleic acids is discussed.
Related Concept Videos
DNA Bacteriophages
Viral Replication: Lytic Cycle
DNA Isolation
Lytic Cycle of Bacteriophages

