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Related Experiment Video

Updated: Jun 5, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

Gene targeting in human pluripotent cells.

D Hockemeyer¹, R Jaenisch

¹Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.

Cold Spring Harbor Symposia on Quantitative Biology

|January 7, 2011

Summary

Efficient gene targeting in human pluripotent stem cells is crucial for biomedical research. Custom-engineered zinc-finger nucleases offer a promising solution for robust genetic modification, advancing disease modeling and therapeutics.

Area of Science:

Stem cell biology
Genetic engineering
Biomedical research

Background:

Mouse embryonic stem cells (mESCs) are vital for biomedical research due to their pluripotency and established gene targeting methods.
Human pluripotent stem cells (hPSCs) hold immense potential for disease modeling and therapeutics, but genetic manipulation is challenging.
Robust genetic tools are needed for hPSCs, mirroring the efficiency seen in mESCs.

Purpose of the Study:

To review strategies for specific genetic modifications in human pluripotent cells.
To highlight the application of custom-engineered zinc-finger nucleases (ZFNs) for gene targeting in hPSCs.

Main Methods:

Discussion of various gene targeting strategies employed in human pluripotent cells.
Focus on the utilization of custom-engineered zinc-finger nucleases (ZFNs).

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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

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Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Related Experiment Videos

Last Updated: Jun 5, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Main Results:

Gene targeting in hPSCs is historically more difficult, time-consuming, and less efficient than in mESCs.
Custom-engineered ZFNs show promise as a robust tool for efficient genetic manipulation of hPSCs.

Conclusions:

Developing efficient gene targeting methods for hPSCs is essential to unlock their full potential.
Custom-engineered ZFNs represent a significant advancement for genetic manipulation in human pluripotent stem cells, paving the way for novel disease models and therapies.