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Updated: Jun 20, 2026

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
Published on: March 18, 2019
[Effects of tumor necrosis factor-alpha on the growth of rat osteoblasts]
Li-wei Xue1, Jun Zhang, Xu-xia Wang
1Dept. of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan 250012, China.
Objective:
To investigate the effects of tumor necrosis factor alpha (TNF-alpha) on the growth of rat osteoblasts. To find out the mechanisms that TNF-alpha regulates the growth of osteoblasts.
Methods:
To assay osteoblasts proliferation by MTT. To assay alkaline phosphatase (ALP) activity of osteoblasts by PP-nitrophenyl phosphate (PNPP).
Results:
The osteblasts proliferation and the ALP activity decreased in treatment groups, and the significantly lower levels were observed in above 50 ng/mL groups (P<0.05).
Conclusion:
TNF-alpha restrained osteoblasts proliferation and differentiation, and the effects were more significant in above 50 ng/mL groups.
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