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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Published on: July 17, 2018
Correlation of rapid phenotypic changes and insulin production of differentiated human adipose tissue-derived stem
Thomas Scholz1, Sapna Satyanarayan, Sanjay Dhar
1Aesthetic and Plastic Surgery Institute, University of California, Irvine, Orange, CA 92868, USA. tscholz@uci.edu
Abstract:
The study investigates adipose tissue-derived stem cells (ADSCs) to differentiate into insulin-secreting cells in vitro. ADSCs were exposed to differentiation medium or control medium. Culture medium was harvested for quantification of insulin and cells were classified into stages of differentiation ranging from normal appearance to islet-like clusters. Morphologic analysis demonstrated marked phenotypic changes towards the islet cell lineage. Thirty-six percent of cells exposed to differentiation medium had progressed to morphologic end-stage after 15 days. Chemiluminescence of the culture medium determined that insulin secretion by differentiated cells progressively increased, reaching a maximum at day 7. Insulin secretion by control cells was significantly less at all time points. A high correlation of secreted insulin and the presence of stage 3 cells were observed throughout the entire experiment. ADSCs can be differentiated into insulin-secreting cells in response to defined culture conditions. The secreted insulin significantly correlates with phenotypic changes throughout the differentiation process.