Distinct changes in soybean xylem sap proteome in response to pathogenic and symbiotic microbe interactions
Senthil Subramanian1, Un-Haing Cho, Carol Keyes
1Donald Danforth Plant Science Center, St Louis, MO, 63132, USA. Senthil.Subramanian@sdstate.edu
BMC Plant Biology
|September 24, 2009
Summary
Soybean xylem sap contains proteins that change in response to pathogen and symbiont interactions. These plant systemic signaling molecules are crucial for understanding root-microbe communication.
Area of Science:
- Plant biology
- Proteomics
- Plant-microbe interactions
Background:
- Systemic signaling in plants involves long-distance transport of molecules via xylem and phloem.
- Root-microbe interactions generate signals transported through xylem sap to aerial organs.
Purpose of the Study:
- Analyze the soybean xylem sap proteome in response to pathogenic and symbiotic interactions.
- Identify systemic signaling proteins and differentially expressed proteins.
Main Methods:
- Proteomic analysis of soybean xylem sap.
- Treatment with Phytophthora sojae elicitor and inoculation with Bradyrhizobium japonicum.
- In vitro growth assays with fungal pathogen Neurospora crassa.
- RNAi-mediated gene silencing.
Main Results:
- Increased serine protease and peroxidase in xylem sap after P. sojae elicitor treatment.
- Soybean xylem sap fraction modulated N. crassa growth, with enhanced inhibition from pathogen-treated sap.
- Increased xyloglucan transendoglycosyl transferase protein abundance after B. japonicum inoculation.
- Gene silencing did not significantly affect soybean nodulation.
Conclusions:
- Identified differentially induced sap proteins in soybean in response to B. japonicum and P. sojae elicitor treatments.
- A majority of the identified proteins were secreted proteins.
More Related Videos
Related Concept Videos
01:09Microbe-Plant Interactions
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
02:26Defenses Against Pathogens and Herbivores
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
01:26Microbial Interactions: Cooperation
Microbial cooperation involves beneficial interactions in which different species work together for individual or mutual advantage. These interactions can profoundly influence ecological dynamics and evolutionary processes, and they are essential to many pathogenic and symbiotic relationships.Nematode–Bacteria CooperationA striking example is the relationship between the Gram-negative bacterium Xenorhabdus nematophila and the parasitic nematode Steinernema carpocapsae. Juvenile nematodes...