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On-line microdialysis coupled with liquid chromatography for biomedical analysis
Guan-Wen Cheng1, Keng-Chang Hsu, Cheng-Fa Lee
1Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Journal of Chromatographic Science
|September 24, 2009
Summary
Microdialysis coupled with High-Performance Liquid Chromatography (MD-HPLC) offers efficient on-line analysis of biological fluids. This review highlights its effectiveness for continuous monitoring in biomedical research and diagnostics.
Area of Science:
- Biomedical Analysis
- Analytical Chemistry
- Neuroscience
Background:
- Microdialysis (MD) is a sampling technique used for in vivo analysis.
- High-Performance Liquid Chromatography (HPLC) is a powerful separation technique.
- On-line coupling of MD with HPLC (MD-HPLC) has evolved for biomedical applications.
Purpose of the Study:
- To review the microdialysis (MD) sampling technique for on-line coupling with HPLC for biomedical analysis.
- To outline the effectiveness of continuous monitoring of unbound chemicals from tissues, organs, and body fluids by on-line MD-HPLC methods.
- To discuss the application of in vivo on-line MD-HPLC for obtaining biochemical event information.
Main Methods:
- Review of existing literature on microdialysis coupled with HPLC.
- Focus on on-line MD-HPLC hyphenated methods.
- Discussion of applications in studying brain metabolism, neurotransmission, and extracellular fluid analysis.
Main Results:
- On-line MD-HPLC offers advantages over off-line techniques: simplified sample preparation, automated analysis, reduced contamination, and in situ analysis.
- Effective continuous monitoring of unbound chemicals from various biological sources is achievable.
- MD-HPLC enables in vivo analysis of extracellular fluid for diverse research areas.
Conclusions:
- On-line MD-HPLC is a valuable technique for continuous, in situ monitoring of biochemical events in living organisms.
- Its applications span pharmacokinetic, pharmacodynamic, toxicological studies, and bioprocess monitoring.
- The technique provides crucial insights into extracellular fluid composition and dynamics.
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