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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence of turnip ringspot virus
1Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of Academy of Sciences of the Czech Republic, v.v.i., Branisovská, Ceské Budejovice, Czech Republic.
Abstract:
Here we present the complete genome sequences of two TuRSV isolates. They are 90-100% identical in distinct genes, but reasonably less identical with RaMV isolates. Regarding the CPs, TuRSV and RaMV have an aa sequence identity of 72-74% among all isolates and the proposed cut-off level is 75%. For the proteinase-polymerase region, the average value between the two isolates of TuRSV and three isolates of RaMV is 79.8% and the cut-off level is 80%. At the moment, TuRSV and RaMV are the two identified species most closely related within the genus Comovirus.
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