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Electrospun polyethersulfone affinity membrane: membrane preparation and performance evaluation.

Zuwei Ma¹, Zhengwei Lan, Takeshi Matsuura

¹Nanoscience and Nanotechnology Initiative, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore. maz@upmc.edu

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|September 25, 2009

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Electrospun polyethersulfone (PES) affinity membranes were developed for protein purification. While showing limitations in large-scale applications, these membranes offer efficient IgG binding for fast, small-scale purification.

Area of Science:

Biomaterials Science
Chemical Engineering
Separation Science

Background:

Developing efficient affinity membranes is crucial for protein purification.
Electrospinning offers a versatile method for creating novel membrane structures.

Purpose of the Study:

To prepare and characterize electrospun polyethersulfone (PES) affinity membranes.
To evaluate their binding efficiency for protein purification, particularly for immunoglobulin G (IgG).

Main Methods:

Membrane preparation via electrospinning, followed by heat treatment and surface functionalization with ligands.
Characterization of membrane properties (fiber diameter, porosity, pore size, ligand density).
Dynamic adsorption studies using bovine serum albumin (BSA) and immunoglobulin G (IgG), analyzed with breakthrough curves and SDS-PAGE.

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Main Results:

High plate height (1.6-8 cm) indicated poor dynamic binding efficiency for BSA, attributed to material microstructure.
PES affinity membranes demonstrated specific IgG binding with high selectivity and low non-specific adsorption.
Comparable IgG binding capacity (4.5 mg/ml) to existing membranes, with a lower pressure drop due to larger pore size.

Conclusions:

Electrospun PES affinity membranes are suitable for fast, small-scale protein purification, especially for IgG.
These membranes show promise as spin column packing materials for rapid protein purification applications.
Further optimization may be needed for large-scale preparative chromatography.