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Updated: Jun 20, 2026

In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
PTH-induced internalization of apical membrane NaPi2a: role of actin and myosin VI
Judith Blaine1, Kayo Okamura, Hector Giral
1Department of Medicine, University of Colorado Denver, Aurora, 80045, USA. Judith.Blaine@ucdenver.edu
Abstract:
Parathyroid hormone (PTH) plays a critical role in the regulation of renal phosphorous homeostasis by altering the levels of the sodium-phosphate cotransporter NaPi2a in the brush border membrane (BBM) of renal proximal tubular cells. While details of the molecular events of PTH-induced internalization of NaPi2a are emerging, the precise events governing NaPi2a removal from brush border microvilli in response to PTH remain to be fully determined. Here we use a novel application of total internal reflection fluorescence microscopy to examine how PTH induces movement of NaPi2a out of brush border microvilli in living cells in real time. We show that a dynamic actin cytoskeleton is required for NaPi2a removal from the BBM in response to PTH. In addition, we demonstrate that a myosin motor that has previously been shown to be coregulated with NaPi2a, myosin VI, is necessary for PTH-induced removal of NaPi2a from BBM microvilli.
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