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[Screening and identification of polypeptides specifically binding to human B type natriuretic peptide from 12TM
Zhen-hui Zhang1, Shi-ming Liu, Wen-yan Wu
1Intensive Care Unit, Second Affiliated Hospital of Guangzhou Medical College, Guangzhou, China. drzhzhh@126.com
Objective:
To identify and characterize the polypeptides specifically binding to human B type natriuretic peptide (BNP) screened from 12TM phage display peptide library.
Methods:
The BNP-binding peptides were screened from 12TM phage display peptide library and identified by ELISA.
Results:
After 4 rounds of screening, 10 of the 16 phage clones were identified as the positive clones which could bind to BNP. Five amino acid sequences were obtained in the 10 positive clones. Dose-dependent ELISA results demonstrated that the screened polypeptides could specifically bind to BNP.
Conclusion:
These screened polypeptides can bind specifically to BNP, which provides a basis for further research on expression and purification of anti-BNP polypeptides and the development of the detection kit of BNP.
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