Comparison of ePTFE graft repair with primary suturing in an experimental duodenal perforation model in rats

In this experimental study, we treated peptic ulcer perforation in the first portion of the duodenum using an ePTFE (expanded polytetrafluoroethylene) soft tissue graft and compared results with primary suturing.

Wistar Albino rats were randomized into two groups. A duodenal defect of 3 mm was created in the first portion of the duodenum in all 24 rats. Macroscopic evaluation was done to determine presence of intraabdominal leakage, peritonitis, intraabdominal adhesions, and the level of adhesion, if present. The adhesion severity scoring system was used to score severity of adhesions. Histology section score criteria were used for scoring in microscopic evaluation.

There was no statistically significant difference between the two groups in the adhesion severity scoring according to grades. However, when overall scoring was considered, a statistically significant difference was determined between the two groups (p=0.045). According to microscopic examination, there was no statistically significant difference between the two groups when classified into none to minimal cell accumulation, thin immature granulations and moderately thick granulation (p=0.089, p=0.178, p=0.755); however, there was a statistically significant difference between the two groups in the thick vascular granulation class (p=0.005).

Use of the ePTFE graft method is easy, and results are comparable to those achieved with primary suturing. However, our results need further confirmation with larger series in animals and also with human studies.