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Related Experiment Video

Updated: Jun 20, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Ultrahigh-throughput FACS-based screening for directed enzyme evolution.

Guangyu Yang1, Stephen G Withers

  • 1Centre for High-Throughput Biology (CHiBi) and Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada.

Chembiochem : a European Journal of Chemical Biology
|September 26, 2009
PubMed
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Directed enzyme evolution uses screening to improve enzymes. Fluorescence-activated cell sorting (FACS) enhances this process, enabling rapid analysis of millions of enzyme variants for better activity and novel functions.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Directed enzyme evolution is key for enzyme property improvement.
  • Screening strategy efficiency is critical for success in enzyme evolution.
  • Fluorescence-activated cell sorting (FACS) offers high sensitivity and throughput for screening.

Purpose of the Study:

  • To review FACS-based screening for enzymatic activity.
  • To discuss the application of FACS in directed evolution.
  • To highlight FACS for enzymes, ribozymes, and catalytic antibodies.

Main Methods:

  • Utilizing FACS for high-throughput screening of enzyme libraries.
  • Analyzing up to 10(8) enzyme mutants daily.
  • Employing FACS for isolating enzyme variants with desired properties.

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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Related Experiment Videos

Last Updated: Jun 20, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Main Results:

  • FACS enables isolation of enzyme variants with enhanced activities.
  • FACS facilitates the discovery of altered substrate specificities.
  • FACS aids in identifying enzyme variants with novel functions.

Conclusions:

  • FACS is a powerful tool for screening enzyme libraries in directed evolution.
  • FACS significantly improves the efficiency of enzyme engineering.
  • FACS has broad applications for evolving enzymes, ribozymes, and catalytic antibodies.