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Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID

Xiaomei Bie1, Zhaoxin Lu, Fengxia Lu

  • 1College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang Nanjing 210095, PR China.

Journal of Microbiological Methods
|September 29, 2009
PubMed

Insights

Bacillus subtilis fmbJ produces fengycin lipopeptides. Electrospray ionization mass spectrometry/collision-induced dissociation (ESI-MS/CID) identified unique ions as fingerprints for rapid fengycin A and B detection.

Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Fengycin, a lipopeptide antibiotic, is produced by Bacillus subtilis.
  • Characterizing fengycin homologues is crucial for understanding their biological activity and production.
  • Rapid detection methods are needed for identifying fengycin-producing strains.

Purpose of the Study:

  • To develop a rapid method for detecting fengycin A and fengycin B.
  • To characterize fengycin homologues produced by Bacillus subtilis fmbJ.
  • To establish diagnostic fingerprints for fengycin identification.

Main Methods:

  • Production of fengycin by Bacillus subtilis fmbJ.
  • Purification of fengycin using acid precipitation and methanol extraction.
  • Analysis of fengycin mixture using Electrospray Ionization Mass Spectrometry/Collision-Induced Dissociation (ESI-MS/CID).

Main Results:

  • Specific production ions (m/z 1080, 966, 1108, 994) were identified in ESI-MS/CID spectra.
  • These ions serve as reliable fingerprints for detecting fengycin A and B.
  • Six fengycin A homologues (C14-C19) and four fengycin B homologues (C14-C17) were characterized.

Conclusions:

  • ESI-MS/CID provides a rapid and effective method for detecting fengycin A and B.
  • The identified production ions are valuable fingerprints for fengycin analysis.
  • This method facilitates the rapid determination of fengycin homologue production in microbial strains.

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