Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular mapping of the mouse db mutation.

N Bahary1, R L Leibel, L Joseph

  • 1Laboratory of Molecular Cell Biology, Rockefeller University, New York, NY 10021.

Proceedings of the National Academy of Sciences of the United States of America
|November 1, 1990
PubMed
Summary

The diabetes (db) gene mutation in mice causes obesity and insulin resistance. Genetic mapping identified linked genes, suggesting a conserved syntenic group with human chromosome 1p, and revealed polygenic factors influencing diabetes development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insulin Resistance: Hepatic Molecular Switches Gone Wrong?: Foxa2 Regulates Lipid Metabolism and Ketogenesis in the Liver during Fasting and in Diabetes. Nature 432: 1027-1032, 2004.

Journal of the American Society of Nephrology : JASN·2023
Same author

The long-term impact of receiving incidental findings on parents undergoing genome-wide sequencing.

Journal of genetic counseling·2022
Same author

Long term outcomes of biomaterial-mediated repair of focal cartilage defects in a large animal model.

European cells & materials·2021
Same author

Assessing Shared Decision-Making Clinical Behaviors Among Genetic Counsellors.

Journal of genetic counseling·2018
Same author

Creation of an international registry to support discovery in schwannomatosis.

American journal of medical genetics. Part A·2016
Same author

Leptin receptor mutations in 129 db (3J)/db(3.J) mice and NIH fa (cp)/fa(cp) rats.

Mammalian genome : official journal of the International Mammalian Genome Society·2016

Area of Science:

  • Genetics
  • Molecular Biology
  • Endocrinology

Background:

  • The mouse diabetes (db) mutation causes obesity, hyperphagia, and insulin resistance.
  • Understanding the genetic basis of db is crucial for metabolic disease research.

Purpose of the Study:

  • To clone the db gene and create a molecular map of the surrounding region.
  • To investigate the genetic linkage of db with other markers.
  • To explore factors contributing to diabetes development in obese mice.

Main Methods:

  • Established two genetic crosses: an intraspecific backcross and an interspecific intercross.
  • Genotyped progeny for the db locus and mapped restriction fragment length polymorphisms (RFLPs).
  • Measured body weight, length, and plasma glucose and insulin levels.

Related Experiment Videos

Main Results:

  • Generated a gene order: centromere-Mt4-P lambda Mm3(2)-Ifa-Cjun-db-D4Rp1-Glut1-Mtv-13-Lck.
  • Identified linkage between db and genes such as Cjun, Glut1, and Lck, which map to human chromosome 1p.
  • Observed varied glucose and insulin levels in obese progeny, with some developing overt diabetes and others remaining euglycemic.

Conclusions:

  • The db locus is part of a syntenic group conserved between mouse chromosome 4 and human chromosome 1p.
  • Polygenic factors likely contribute to the development of overt diabetes in obese mice.
  • Further research into these linked genes and polygenic influences is warranted.