Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Evidence that dopamine-2 mechanisms control renal function.

H M Siragy¹, R A Felder, N L Howell

¹Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville 22908.

The American Journal of Physiology

|November 1, 1990

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transient depletion of macrophages alters local inflammatory response at the site of disc herniation in a transgenic mouse model.

Osteoarthritis and cartilage·2023

Same author

New imaging tools to measure nephron number <i>in vivo</i>: opportunities for developmental nephrology.

Journal of developmental origins of health and disease·2020

Same author

Dopaminergic Immunofluorescence Studies in Kidney Tissue.

Methods in molecular biology (Clifton, N.J.)·2017

Same author

A population of mitochondrion-rich cells in the pars recta of mouse kidney.

Cell and tissue research·2015

Same author

Common variants of the G protein-coupled receptor type 4 are associated with human essential hypertension and predict the blood pressure response to angiotensin receptor blockade.

The pharmacogenomics journal·2015

Same author

Glomerulotubular disconnection in neonatal mice after relief of partial ureteral obstruction.

Kidney international·2007

Same journal

Blood coagulation in fish.

The American journal of physiology·2011

Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010

Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010

Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010

Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010

Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010

See all related articles

Intrarenal dopamine acts on dopamine-2 (DA2) receptors in the kidney to regulate renal function. Blocking these DA2 receptors with YM-09151 caused significant changes in urine output and sodium excretion.

Area of Science:

Nephrology
Pharmacology
Physiology

Background:

Dopamine is synthesized within the kidney, and dopamine-2 (DA2) receptors are found in the renal glomerulus.
The specific role of DA2 receptors in kidney function remains undefined.

Purpose of the Study:

To investigate the potential role of DA2 receptors in controlling renal function.
To determine the effects of blocking DA2 receptors on kidney hemodynamics and excretory function.

Main Methods:

Intrarenal infusion of a specific DA2 antagonist, YM-09151, in conscious uninephrectomized dogs.
Dose-dependent administration of YM-09151 and assessment of urinary flow rate, sodium excretion, glomerular filtration rate, and renal plasma flow.
Evaluation of concurrent administration of a DA2 agonist, LY-171555, to confirm receptor specificity.

Related Experiment Videos

Main Results:

YM-09151 induced a significant, dose-dependent increase in diuresis and natriuresis.
Glomerular filtration rate, renal plasma flow, and filtration fraction were significantly elevated by YM-09151.
Coinfusion with a DA2 agonist abolished the effects of YM-09151, confirming DA2 receptor involvement.
No significant changes in plasma renin activity, aldosterone, or mean arterial blood pressure were observed.

Conclusions:

Intrarenally produced dopamine likely acts on renal vascular and/or glomerular DA2 receptors to modulate renal function.
DA2 receptors play a critical role in regulating kidney hemodynamics and sodium excretion.