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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Related Experiment Video

Updated: Jun 8, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

A fluorescence lifetime imaging scanning confocal endomicroscope.

Gordon T Kennedy, Hugh B Manning, Daniel S Elson

    Journal of Biophotonics
    |September 30, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new fluorescence lifetime imaging endomicroscope using a fiber bundle probe and time-correlated single photon counting. This advanced microscopy technique visualizes biological samples like pollen and cells with high detail.

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    Last Updated: Jun 8, 2026

    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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    Published on: February 9, 2012

    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    Fluorescence Lifetime Macro Imager for Biomedical Applications
    06:01

    Fluorescence Lifetime Macro Imager for Biomedical Applications

    Published on: April 7, 2023

    Area of Science:

    • Biomedical Engineering
    • Optical Microscopy
    • Cell Biology

    Background:

    • Fluorescence lifetime imaging (FLIM) provides molecular contrast beyond intensity.
    • Endomicroscopy enables in-situ imaging of biological tissues.
    • Integrating FLIM with endomicroscopy offers enhanced diagnostic capabilities.

    Discussion:

    • The developed system utilizes a fiber bundle probe for endomicroscopy.
    • Time-correlated single photon counting (TCSPC) is employed for precise lifetime measurements.
    • This combination allows for high-resolution, in-vivo fluorescence lifetime imaging.

    Key Insights:

    • Demonstrated preliminary imaging of stained pollen grains.
    • Visualized Förster resonant energy transfer (FRET) in eGFP-labeled cells.
    • Successfully captured endogenous tissue autofluorescence with FLIM endomicroscopy.

    Outlook:

    • Potential for label-free imaging of tissue states.
    • Applications in disease diagnosis and surgical guidance.
    • Further optimization for in-vivo cellular and molecular analysis.