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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

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Updated: Jun 20, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Conditional RNA interference mediated by allosteric ribozyme.

Deepak Kumar¹, Chung-Il An, Yohei Yokobayashi

¹University of California, Davis, Department of Biomedical Engineering 451 Health Sciences Drive, Davis, California 95616, USA.

Journal of the American Chemical Society

|October 1, 2009

Summary

This summary is machine-generated.

Researchers developed a novel RNA system for precise gene silencing control in mammalian cells. This chemical-inducible RNA interference (RNAi) method offers a modular and adaptable approach for regulating gene expression.

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Nanomanipulation of Single RNA Molecules by Optical Tweezers

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Published on: August 20, 2014

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Nanomanipulation of Single RNA Molecules by Optical Tweezers

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Area of Science:

Molecular Biology
Biotechnology
Gene Regulation

Background:

Conditional RNA interference (RNAi) allows controlled gene silencing.
Existing methods often require complex genetic modifications, limiting their application.
There is a need for simpler, chemically inducible RNAi systems.

Purpose of the Study:

To design a novel RNA architecture for chemical induction of RNAi in mammalian cells.
To create a compact and modular system for conditional gene silencing.
To enable RNAi systems that can respond to various molecular signals.

Main Methods:

Designed a novel RNA construct combining a drug-inducible allosteric ribozyme and a microRNA precursor analogue.
Utilized chemical induction for RNAi activation in mammalian cells.
Demonstrated the modularity of the RNA design for potential adaptation.

Main Results:

Successfully developed a chemically inducible RNAi system in mammalian cells.
The novel RNA architecture provides a compact and modular design.
The system is adaptable for sensing and responding to various molecules via RNA aptamers.

Conclusions:

The new RNA design enables precise chemical control over gene silencing.
This modular system facilitates the construction of versatile conditional RNAi applications.
The approach offers a promising tool for regulating gene expression in research and potentially therapeutics.