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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera

Mitri K Khoury1, Ian Parker, Dana W Aswad

  • 1Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA.

Analytical Biochemistry
|October 1, 2009
PubMed

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...