[Guanylate cyclase from human platelets in pulmonary tuberculosis]

Activity of soluble guanylate cyclase and activating effect of sodium nitroprusside were studied in thrombocytes of patients with infiltrative form of pulmonary tuberculosis before treatment (as soon as patients entered the hospital) and within various periods of the antibacterial therapy course: during the first month, 1.5-2, 2.5-3.5 and more than 4 months of treatment. Before treatment activity of guanylate cyclase in the patients thrombocytes was similar to that of donors independently on severity of the process in lungs (impairment of more or less two lung segments). During the antibacterial therapy the enzyme activation was detected, which reached 160% of the normal level within 2 months course and decreased down to the control rate within 4 months of treatment. The activating effect of sodium nitroprusside was distinctly decreased (by 70%) in thrombocytes of patients with infiltrative form of pulmonary tuberculosis before the treatment course, while the pattern was restored to the normal level, comparable with control values in donors, within 4 months of the treatment. The stimulating effect of sodium nitroprusside on guanylate cyclase activity in thrombocytes of patients with infiltrative form of pulmonary tuberculosis may be used as an additional criterion for evaluation of the therapeutic course efficiency.