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Updated: Jun 19, 2026

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
Published on: April 30, 2014
Pax-3 and Pax-7 label muscle progenitor cells during myotomal myogenesis in Coregonus lavaretus (Teleostei:
A Kacperczyk1, T Jagla, M Daczewska
1Department of Animal Developmental Biology, Zoological Institute, University of Wroclaw, Sienkiewicza 21, Wroclaw 50-335, Poland.
Abstract:
In Coregonus lavaretus, prior the mesoderm segmentation, in cells adjacent to the notochord called adaxial cells MyoD and slow myosin heavy chain (MyHC-slow) proteins were observed. After somite formation, adaxial cells migrate towards the lateral part of the myotome and form a layer of red muscles. Deeper cells differentiate into white muscle fibres. In situ hybridization using Pax-3 molecular probe revealed, that after somitogenesis, Pax-3 is expressed in a layer of cells superficial to the myotome resembling the "external cells" (found in many teleosts species) or dermomyotome described in Amniota. During later developmental stages Pax-3 gene is expressed in cells in intermyotomal space and then in myoblasts between myotubes. In these cells Pax-7 protein was also observed. Pax-3/7 positive cells which have migrated into the myotomes differentiate into satellite cells/secondary myoblasts and participate in hypertrophic and hyperplastic growth of muscles.
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