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Updated: Jun 19, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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Nanostructured conducting polymer based reagentless capacitive immunosensor.

Amay Jairaj Bandodkar1, Chetna Dhand, Sunil K Arya

  • 1Department of Applied Chemistry, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.

Biomedical Microdevices
|October 2, 2009
PubMed
Summary
This summary is machine-generated.

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This study developed a novel immunosensor using nanostructured polyaniline (PANI) film for detecting human IgG. The device offers a sensitive and selective method for quantifying human IgG levels.

Area of Science:

  • Electrochemistry
  • Materials Science
  • Biotechnology

Background:

  • Nanostructured polyaniline (PANI) films offer unique electrochemical properties.
  • Indium-tin-oxide (ITO) coated glass is a common substrate for electronic devices.
  • Immunosensors require specific antibody immobilization for target detection.

Purpose of the Study:

  • To develop a novel immunosensor for human IgG detection.
  • To utilize capacitance changes in a parallel plate capacitor (PPC) for sensing.
  • To immobilize anti-human IgG (Anti-HIgG) onto a PANI/ITO platform.

Main Methods:

  • Electrophoretic fabrication of nanostructured PANI film on ITO glass.
  • Covalent immobilization of Anti-HIgG using carbodiimide chemistry.

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  • Characterization via SEM, cyclic voltammetry, DPV, and FTIR.
  • Capacitance measurements for detecting human IgG.

    • Main Results:

    • The PANI/ITO and Anti-HIgG/PANI/ITO platforms were successfully fabricated and characterized.
    • The immunosensor demonstrated sensitivity to human IgG in the range of 5 - 5 x 10^5 ng/mL.
    • A low detection limit of 1.87 ng/mL for human IgG was achieved.

    Conclusions:

    • The developed Anti-HIgG modified PPC functions as a robust and easy-to-use immunosensor.
    • The sensor is reagentless, sensitive, and selective for human IgG estimation.
    • This technology holds promise for accurate human IgG quantification.