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Updated: Jun 19, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
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Two-color subpicosecond optical sampling technique.

J D Kafka, J W Pieterse, M L Watts

    Optics Letters
    |October 3, 2009
    PubMed
    Summary
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    A new laser system enables pump-probe experiments using independently tunable laser pulses. This advanced setup achieves a 150 fs resolution, ideal for ultrafast science research.

    Area of Science:

    • Ultrafast spectroscopy
    • Laser physics
    • Physical chemistry

    Background:

    • Pump-probe spectroscopy is crucial for studying dynamic processes.
    • High temporal resolution is essential for observing rapid molecular events.
    • Existing systems may lack independent tunability or efficient scanning.

    Purpose of the Study:

    • To develop a novel laser system for advanced pump-probe experiments.
    • To achieve high temporal resolution for precise measurements.
    • To enable independent tuning of pump and probe pulses.

    Main Methods:

    • Construction of a laser system utilizing two mode-locked Ti:sapphire lasers.
    • Independent tuning of pump and probe pulse characteristics.
    • Automated time-delay scanning at 80 Hz.

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    Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
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    Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

    Published on: September 5, 2012

    Related Experiment Videos

    Last Updated: Jun 19, 2026

    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
    07:13

    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

    Published on: May 16, 2022

    Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
    08:48

    Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

    Published on: September 5, 2012

  • Measurement of cross-correlation to determine system resolution.

    • Main Results:

    • A laser system capable of performing pump-probe experiments was successfully constructed.
    • The system achieves a temporal resolution of 150 fs.
    • Independent tunability of pump and probe pulses was demonstrated.
    • Automated scanning facilitates efficient data acquisition.

    Conclusions:

    • The developed laser system provides a robust platform for ultrafast pump-probe spectroscopy.
    • The achieved resolution and tunability meet the demands of advanced scientific investigations.
    • This system enhances the capability for studying transient phenomena in various scientific fields.