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Expression of TIP30 tumor suppressor gene is down-regulated in human colorectal carcinoma

Xiaobing Chen1, Xinguang Cao, Wenjie Dong

  • 1Department of Internal Medicine-Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.

Digestive Diseases and Sciences
|October 3, 2009
PubMed
Abstract

Insights

Tumor suppressor gene TIP30 (also known as TIP30) expression is decreased in colorectal carcinoma (CRC). Promoter methylation is a key mechanism driving this reduced TIP30 expression in CRC tumors.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • The human TIP30 gene was initially identified as a potential metastasis suppressor.
  • Down-regulation of TIP30 expression has been observed in various human cancers, including liver, lung, breast, and prostate.
  • Recent investigations have explored the role of TIP30 in colorectal cancer (CRC).

Purpose of the Study:

  • To investigate the expression levels of the TIP30 gene in colorectal carcinoma.
  • To determine the correlation between TIP30 expression and its methylation status in CRC.

Main Methods:

  • Quantitative analysis of TIP30 protein levels in colorectal carcinoma tissues versus normal tissues.
  • Analysis of TIP30 gene promoter methylation frequencies in tumor tissues compared to adjacent non-tumor tissues.
  • Identification of somatic mutations within the TIP30 gene in human CRC specimens.

Main Results:

  • TIP30 protein levels were significantly lower in colorectal carcinomas than in normal tissues.
  • Hypermethylation of the TIP30 gene promoter was detected in 36% of tumors, with no aberrant methylation in paired non-tumor tissues.
  • A significant inverse correlation was observed between TIP30 methylation status and TIP30 protein expression in tumor tissues.
  • Somatic missense mutations in the TIP30 gene were identified in human CRC tissues.

Conclusions:

  • Promoter methylation plays a crucial role in the decreased expression of the TIP30 tumor suppressor gene in human colorectal carcinoma.
  • These findings highlight the involvement of epigenetic alterations in TIP30 down-regulation in CRC.

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